This interactive app offers you a simple way of analyzing and playing with the microbial commuminty data.
You can explore raw data, merge replicate samples, perform state-of-the-art analysis and find interactive visualizations.
To learn how the app works and what you can do watch a short intro video, check the online manual / tutorial or simply explore on your own.
The intro video will show you the most important functions and basic bahavior:
Link to videosThe manual explains the behavior and options of each control element and analysis window.
Link to Manual / TutorialTo detect specialist and generalist different approaches are known. Here indicator species will be detected as specialist. Generalist will be detected by minimal abundance and minimal occurence.
We recommend to restrict the indicator species detection to the top expressed ASV/OTUs only.
Your selection includes the following number of features:
You can select the top x features based on order of your input table. Note: The order of your uploaded data is changed if sorting by mean before selection is active.
You can select most interesting feature by the mean of all samples. To view the distribution of the mean explore the plot below.
There are several concepts of (habitat) specialist or indicator species that slighly differ. Here we use the indicator value detection by Dufrene and Legendre (1997). The analysis requires two inputs: an abundance / count table and groups. The analysis output is two value for each feature (ASV/OTU/MAG): a P-value of statistical significance of enrichment in one of the groups and an indicator value showing the strength of indication for this group (value from 0 to 1), with higher values showing more specific enrichtment for the group. For more examples of how to find specific features or to interprete the results see the tutorial.
Do you want to store your loaded and processed data? This enables automatic loading of the data on next app start.
Calculate relative abundance from raw data.
You can apply a cutoff to remove low abundant ASV's or OTU's, e.g. rare biosphere.
Order samples by drag and drop. Missing samples will be added on saving in there default order.